BTLA active protein

BTLA Protein, Rat, Recombinant (ECD, mFc Tag)

Cat. Num. AAA8123504

• Purity: >90% as determined by SDS-PAGE
• Synonyms: BTLA; BTLA Protein; Rat; Recombinant (ECD; mFc Tag); Rat BTLA Protein (ECD; Fc Tag); B and T lymphocyte associated; BTLA active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala6-Tyr183

• Species: Rat
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 30
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat Btla (NP_998795.1)(Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized rat BTLA-mFc at 10ug/mL (100uL/well) can bind mouse HVEM-Fch. The EC50 of mouse EFNA5-Fc is 6.2-14.4ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BTLA active protein

Background: BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

Product Categories/Family for BTLA active protein

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

References

Fourcade J, et al. (2012) CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72(4):887-96.Kojima R, et al. (2011) Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT. J Mol Biol. 413(4):762-72.Oki M, et al. (2011) A functional polymorphism in B and T lymphocyte attenuator is associated with susceptibility to rheumatoid arthritis. Clin Dev Immunol. 305656.

NCBI and Uniprot Product Information

• NCBI GI #: 47155571
• NCBI GeneID: 407756
• NCBI Accession #: NP_998795.1
• NCBI GenBank Nucleotide #: NM_213630.1
• UniProt Accession #: Q6PNM1; Q569B2
• Molecular Weight: 34,476 Da
• NCBI Official Full Name: B- and T-lymphocyte attenuator
• NCBI Official Synonym Full Names: B and T lymphocyte associated
• NCBI Official Symbol: Btla
• NCBI Protein Information: B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein
• UniProt Protein Name: B- and T-lymphocyte attenuator
• Protein Family: B- and T-lymphocyte attenuator
• UniProt Gene Name: Btla
• UniProt Entry Name: BTLA_RAT

Uniprot Description

Function: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response