KIRREL3 active protein

KIRREL3 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123389

• Purity: >95% as determined by SDS-PAGE
• Synonyms: KIRREL3; KIRREL3 Protein; Rat; Recombinant (His Tag); Rat KIRREL3/NEPH2 Protein (His Tag); kin of IRRE like 3 (Drosophila); KIRREL3 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala523

• Species: Rat
• Activity: Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to rat KIRREL3 coated plates (15ug/mL, 100uL/well), >20% will adhere specifically after 90 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat KIRREL3 (Q09GS6)(Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KIRREL3 active protein

Background: Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in the fetal and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating Nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the Nucleogenesis of the PN through the control of neuronal migration inside the nucleus.

References

Bhalla K, et al. (2008) Alterations in CDH15 and KIRREL3 in patients with mild to severe intellectual disability. Am J Hum Genet. 83(6): 703-13.Gerke P, et al. (2005) NEPH2 is located at the glomerular slit diaphragm, interacts with nephrin and is cleaved from podocytes by metalloproteinases. J Am Soc Nephrol. 16(6): 1693-702.Gerke P, et al. (2006) Neuronal expression and interaction with the synaptic protein CASK suggest a role for Neph1 and Neph2 in synaptogenesis. J Comp Neurol. 498(4): 466-75.

NCBI and Uniprot Product Information

• NCBI GI #: 115292425
• NCBI GeneID: 315546
• NCBI Accession #: NP_001041680.1
• NCBI GenBank Nucleotide #: NM_001048215.1
• UniProt Accession #: Q09GS6
• Molecular Weight: 84,299 Da
• NCBI Official Full Name: kin of IRRE-like protein 3
• NCBI Official Synonym Full Names: kin of IRRE like 3 (Drosophila)
• NCBI Official Symbol: Kirrel3
• NCBI Protein Information: kin of IRRE-like protein 3
• UniProt Protein Name: Membrane protein KIRRE
• Protein Family: Kin of IRRE-like protein
• UniProt Gene Name: Kirrel3