COX5B recombinant protein

COX5B Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121925

• Gene Names: COX5B; COXVB
• Purity: >95% as determined by SDS-PAGE
• Synonyms: COX5B; COX5B Protein; Human; Recombinant (His Tag); Human COX5B Protein (His Tag); COXVB Protein; cytochrome c oxidase subunit Vb; COX5B recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala32-His129

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human COX5B (P10606)(Ala32-His129) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for COX5B recombinant protein

Background: Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

References

Romero N. et al., 1990, Histochemistry. 94 (2): 211-5. Lomax MI. et al., 1991, Genomics. 10 (1): 1-9. Beauchemin A M. et al., 2001, Brain Res Bull. 56 (3-4): 285-97.

NCBI and Uniprot Product Information

• NCBI GI #: 20141332
• NCBI GeneID: 1329
• UniProt Accession #: P10606; Q53YB7; Q96J18; Q99610
• Molecular Weight: 129
• NCBI Official Full Name: Cytochrome c oxidase subunit 5B, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit Vb
• NCBI Official Symbol: COX5B
• NCBI Official Synonym Symbols: COXVB
• NCBI Protein Information: cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase polypeptide VB, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 5B, mitochondrial
• Protein Family: Cytochrome c oxidase polypeptide
• UniProt Gene Name: COX5B
• UniProt Entry Name: COX5B_HUMAN

NCBI Description

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

COX5B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5B family.

Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: cytochrome-c oxidase activity; metal ion binding

Biological Process: cellular metabolic process; respiratory gaseous exchange; transmembrane transport