Immunohistochemistry (IHC)
(Immunochemical staining of mouse KIRREL3 in mouse kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Rabbit anti-Mouse KIRREL3 Polyclonal Antibody | anti-KIRREL3 antibody
Anti-KIRREL3 Antibody, Rabbit Polyclonal
Gene Names
Kirrel3; SST4; NEPH2; mKIAA1867; 1500010O20Rik; 2900036G11Rik
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
KIRREL3; Polyclonal Antibody; Anti-KIRREL3 Antibody; Rabbit Polyclonal; KIRREL3/NEPH2 Antibody; Rabbit PAb; Antigen Affinity Purified; kin of IRRE like 3 (Drosophila); Anti-1500010O20Rik Antibody; Anti-2900036G11Rik Antibody; Anti-mKIAA1867 Antibody; Anti-NEPH2 Antibody; Anti-SST4 Antibody; anti-KIRREL3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse KIRREL3/NEPH2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-KIRREL3 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:2500-1:10,000
IHC-P: 1:2500-1:10,000
Immunogen
Recombinant Mouse KIRREL3/NEPH2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse KIRREL3/NEPH2 (rM KIRREL3/NEPH2). KIRREL3/NEPH2 specific IgG was purified by Mouse KIRREL3/NEPH2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KIRREL3 in mouse kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse KIRREL3 in mouse kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Related Product Information for anti-KIRREL3 antibody
Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in the fetal and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size-and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating Nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the Nucleogenesis of the PN through the control of neuronal migration inside the nucleus.
References
Bhalla K, et al. (2008) Alterations in CDH15 and KIRREL3 in patients with mild to severe intellectual disability. Am J Hum Genet. 83(6): 703-13.Gerke P, et al. (2005) NEPH2 is located at the glomerular slit diaphragm, interacts with nephrin and is cleaved from podocytes by metalloproteinases. J Am Soc Nephrol. 16(6): 1693-702.Gerke P, et al. (2006) Neuronal expression and interaction with the synaptic protein CASK suggest a role for Neph1 and Neph2 in synaptogenesis. J Comp Neurol. 498(4): 466-75.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,405 Da
NCBI Official Full Name
kin of IRRE-like protein 3 isoform A
NCBI Official Synonym Full Names
kin of IRRE like 3 (Drosophila)
NCBI Official Symbol
Kirrel3
NCBI Official Synonym Symbols
SST4; NEPH2; mKIAA1867; 1500010O20Rik; 2900036G11Rik
NCBI Protein Information
kin of IRRE-like protein 3; X kin of IRRE like 3; membrane protein mKirre; kin of irregular chiasm-like protein 3
UniProt Protein Name
Kin of IRRE-like protein 3
Protein Family
UniProt Gene Name
Kirrel3
UniProt Synonym Gene Names
Kiaa1867
UniProt Entry Name
KIRR3_MOUSE
Similar Products
Product Notes
The KIRREL3 kirrel3 (Catalog #AAA8111414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KIRREL3 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIRREL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:2500-1:10,000. Researchers should empirically determine the suitability of the KIRREL3 kirrel3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIRREL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
