CNTF active protein

CNTF Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123197

• Gene Names: Cntf; AI429687
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CNTF; CNTF Protein; Mouse; Recombinant (His Tag); Mouse CNTF/Ciliary Neurotrophic Factor Protein (His Tag); AI429687 Protein; ciliary neurotrophic factor; CNTF active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Met198

• Species: Mouse
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse CNTF (NP_740756.1)(Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10ug/ml (100uL/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNTF active protein

Background: Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFalpha) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.

References

Dutt K, et al. (2010) Ciliary neurotrophic factor: a survival and differentiation inducer in human retinal progenitors. In Vitro Cell Dev Biol Anim. 46 (7) : 635-46.Lam A, et al.(1991) Sequence and structural organization of the human gene encoding ciliary neurotrophic factor. Gene 102 (2) : 271-6.Bazan JF. (1991) Neuropoietic cytokines in the hematopoietic fold. Neuron 7 (2) : 197-208.

NCBI and Uniprot Product Information

• NCBI GI #: 25952141
• NCBI GeneID: 12803
• NCBI Accession #: NP_740756.1
• NCBI GenBank Nucleotide #: NM_170786.2
• UniProt Accession #: P51642
• Molecular Weight: 22,587 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: Cntf
• NCBI Official Synonym Symbols: AI429687
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: Cntf
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_MOUSE

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Cellular Component: extracellular space; cell soma; axon; cytoplasm; nucleus; cytosol

Molecular Function: interleukin-6 receptor binding; growth factor activity; cytokine activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; nervous system development; positive regulation of axon regeneration; protein heterooligomerization; multicellular organismal development; membrane hyperpolarization; positive regulation of hormone secretion; positive regulation of JAK-STAT cascade; JAK-STAT cascade; positive regulation of cell growth; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; regulation of resting membrane potential; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; astrocyte activation; cell differentiation; muscle morphogenesis; growth