KIRREL Active Protein | KIRREL1 active protein

KIRREL Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121954

• Gene Names: KIRREL; NEPH1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: KIRREL; KIRREL Protein; Human; Recombinant (His Tag); Human KIRREL1/NEPH1 Protein (His Tag); NEPH1 Protein; kin of IRRE like (Drosophila); KIRREL1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu493

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15ug/mL, 100uL/well), >20% will adhere specifically after 90 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human KIRREL (NP_060710.3)(Met1-Leu493) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KIRREL1 active protein

Background: NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain, and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation of the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice result in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

References

Sellin L, et al. (2003) NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 17(1): 115-7.Kim EY, et al. (2009) Neph1 regulates steady-state surface expression of Slo1 Ca(2+)-activated K(+) channels: different effects in embryonic neurons and podocytes. Am J Physiol Cell Physiol. 297(6): C1379-88.

NCBI and Uniprot Product Information

• NCBI GI #: 166295173
• NCBI GeneID: 55243
• NCBI Accession #: NP_060710.3
• NCBI GenBank Nucleotide #: NM_018240.6
• UniProt Accession #: Q80W68; Q5W0F8; Q5XKC6; Q7Z696; Q7Z7N8; Q8TB15; Q9H9N1; Q9NVA5; Q96J84
• Molecular Weight: 72,360 Da
• NCBI Official Full Name: kin of IRRE-like protein 1 isoform 1
• NCBI Official Synonym Full Names: kin of IRRE like (Drosophila)
• NCBI Official Symbol: KIRREL
• NCBI Official Synonym Symbols: NEPH1
• NCBI Protein Information: kin of IRRE-like protein 1
• UniProt Protein Name: Kin of IRRE-like protein 1
• UniProt Gene Name: Kirrel
• UniProt Synonym Gene Names: Kirrel1; Neph1
• UniProt Entry Name: KIRR1_MOUSE

NCBI Description

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Uniprot Description

KIRREL: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. Belongs to the immunoglobulin superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: cell projection membrane; dendritic shaft; integral to membrane; intercellular junction; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: myosin binding; protein binding

Biological Process: cell-cell adhesion; excretion; negative regulation of protein amino acid phosphorylation; positive regulation of actin filament polymerization