ICOSLG/B7-H2/CD275 Active Protein | ICOSLG active protein

Human CellExp ICOSLG / B7-H2 / CD275, human recombinant

Cat. Num. AAA844797

• Gene Names: ICOSLG; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: ICOSLG/B7-H2/CD275; Human CellExp ICOSLG / B7-H2 / CD275; human recombinant; ICOSLG; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; ICOS ligand.; ICOSLG active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 309

• Applicable Applications for ICOSLG active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 mug/ml (100 mul/well) can bind human ICOS with a linear range of 3.2 - 400 ng/ml.
• Results: Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 mug/ml (100 mul/well) can bind human ICOS with a linear range of 3.2 - 400 ng/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for ICOSLG active protein

Background: ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

Product Categories/Family for ICOSLG active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 545688560
• NCBI GeneID: 23308
• NCBI Accession #: NP_001269979.1
• NCBI GenBank Nucleotide #: NM_001283050.1
• UniProt Accession #: O75144; Q9HD18; Q9NRQ1; A8MUZ1
• Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.5 kDa. The predicted N-terminus is Asp 19. DTT-reduced Protein migrates as 45-50 kDa due to glycosylation.
• NCBI Official Full Name: ICOS ligand isoform b
• NCBI Official Synonym Full Names: inducible T-cell co-stimulator ligand
• NCBI Official Symbol: ICOSLG
• NCBI Official Synonym Symbols: B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
• NCBI Protein Information: ICOS ligand
• UniProt Protein Name: ICOS ligand
• Protein Family: ICOS ligand
• UniProt Gene Name: ICOSLG
• UniProt Synonym Gene Names: B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1
• UniProt Entry Name: ICOSL_HUMAN

Uniprot Description

ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: receptor binding

Biological Process: hyperosmotic response; T cell activation; B cell activation; T cell costimulation; defense response; signal transduction; positive regulation of activated T cell proliferation