Mouse anti-Human, Rat XRCC1 Monoclonal Antibody | anti-XRCC1 antibody

XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free) (HRP)

Cat. Num. AAA6266842

• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRCC1; Monoclonal Antibody; XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free) (HRP); anti-XRCC1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3F399
• Specificity: Recognizes XRCC1 (X-Ray Repair Cross Complementing Protein1). Species Crossreactivity: human and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-XRCC1 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant human XRCC1 protein
• Conjugate: HRP
• Preparation and Storage: Store at -20 degree C.

Testing Data

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Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.)

Product Categories/Family for anti-XRCC1 antibody

Antibodies; Transcription Factors

References

1. Cappelli E; et al. J Bio Chem, 1997, 272:23970-5. 2. Labudova O; et al. Pediatric Res, 1997, 41:435-9. Additional: 1. Nash RA; Caldecott KW; Barnes DE; Lindahl T. XRCC1 protein interacts with one of two distinct forms of DNA ligase III. Biochemistry, 1997, 36(17):5207-11. 2. Andersson BS; Sadeghi T; Siciliano MJ; Legerski R; Murray D. Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs. Cancer Chemotherapy and Pharmacology, 1996, 38(5):406-16. 3. Caldecott KW; Aoufouchi S; Johnson P; Shall S. XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro. Nucleic Acids Research, 1996, 24:4387-94. 4. Kubota Y; Nash RA; Klungland A; Schar P; Barnes DE; Lindahl T. Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein. Embo Journal, 1996, 15:6662-70. 5. Walter CA; Trolian DA; McFarland MB; Street KA; Gurram GR; McCarrey JR. Xrcc-1 expression during male meiosis in the mouse. Biology of Reproduction, 1996 Sep, 55(3):630- 5. 6. Caldecott KW; Tucker JD; Stanker LH; Thompson LH. Characterization of the XRCC1- DNA ligase III complex in vitro and its absence from mutant hamster cells. Nucleic Acids Research, 1995, 23(23):4836-43. 7. Lamerdin JE; Montgomery MA; Stilwagen SA; Scheidecker LK; Tebbs RS; Brookman KW; Thompson LH; Carrano AV. Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions. Genomics, 1995, 25(2):547-54. 8. Wei YF; Robins P; Carter K; Caldecott K; Pappin DJ; Yu GL; Wang RP; Shell BK; Nash RA; Schar P; et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Molecular and Cellular Biology, 1995, 15(6):3206-16. 9. Zhou ZQ; Walter CA. Expression of the DNA repair gene XRCC1 in baboon tissues. Mutation Research, 1995 Nov, 348(3):111-6. 10. Brookman KW; Tebbs RS; Allen SA; Tucker JD; Swiger RR; Lamerdin JE; Carrano AV; Thompson LH. Isolation and characterization of mouse Xrcc-1, a DNA repair gene affecting ligation. Genomics, 1994 Jul 1, 22(1):180-8. 11. Caldecott KW; McKeown CK; Tucker JD; Ljungquist S; Thompson LH. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III. Molecular and Cellular Biology, 1994, 14(1):68-76. 12. Caldecott KW; Thompson LH. Partial correction of the single-strand break repair defect in the CHO mutant EM9 by electroporated recombinant XRCC1 protein. Annals of the New York Academy of Sciences, 1994 Jul 29, 726:336-9. 13. Ljungquist S; Kenne K; Olsson L; Sandstrom M. Altered DNA ligase III activity in the CHO EM9 mutant. Mutation Research, 1994, 314(2):177- 86. 14. Shung B; Miyakoshi J; Takebe H. X-rayinduced transcriptional activation of c-myc and XRCC1 genes in ataxia telangiectasia cells. Mutation Research, 1994, 307(1):43-51. 15. Walter CA; Lu J; Bhakta M; Zhou ZQ; Thompson LH; McCarrey JR. Testis and somatic Xrcc-1 DNA repair gene expression. Somatic Cell and Molecular Genetics, 1994, 20(6):451-61. 16. Lehmann AR. Duplicated region of sequence similarity to the human XRCC1 DNA repair gene in the Schizosaccharomyces pombe rad4/cut5 gene. Nucleic Acids Research, 1993, 21(22):5274