ICOS Ligand / B7-H2 / ICOSLG Recombinant Protein | ICOSLG recombinant protein

Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (His tag)

Cat. Num. AAA2545745

• Gene Names: ICOSLG; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: ICOS Ligand / B7-H2 / ICOSLG; Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (His tag); B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; KIAA0653; LICOS; ICOSLG recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 302

• Application Notes: The secreted recombinant human ICOSLG consists of 251 amino acids and has a calculated molecular mass of 28 kDa. The apparent molecular mass of the protein is approximately 50-60 kDa in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Asp 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ICOSLG recombinant protein

Background: Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.

Description: A DNA sequence encoding the human ICOSLG (NP_056074.1) extracellular domain (Met 1-Ser 258) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 27477039
• NCBI GeneID: 23308
• NCBI Accession #: NP_056074.1
• NCBI GenBank Nucleotide #: NM_015259.5
• UniProt Accession #: O75144; Q9HD18; Q9NRQ1; A8MUZ1
• Molecular Weight: 20,120 Da
• NCBI Official Full Name: ICOS ligand isoform a
• NCBI Official Synonym Full Names: inducible T-cell costimulator ligand
• NCBI Official Symbol: ICOSLG
• NCBI Official Synonym Symbols: B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
• NCBI Protein Information: ICOS ligand
• UniProt Protein Name: ICOS ligand
• Protein Family: ICOS ligand
• UniProt Gene Name: ICOSLG
• UniProt Synonym Gene Names: B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1

Uniprot Description

ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: plasma membrane

Molecular Function: receptor binding

Biological Process: positive regulation of activated T cell proliferation; T cell costimulation