LIF active protein

Human CellExp LIF, mouse recombinant

Cat. Num. AAA844783

• Applications: SDS-Page
• Purity: >=92%
• Synonyms: LIF; Human CellExp LIF; mouse recombinant; CDF; DIA; HILDA; MLPLI; LIF active protein

• Host: HEK 293 cells
• Purity/Purification: >=92%
• Form/Format: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 158

• Applicable Applications for LIF active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug) by LAL method
• Activity (Specifications/test method): Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml
• Biological Activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for LIF active protein

Background: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Product Categories/Family for LIF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins (NEW) Cytokines; Growth Factors & Hormones; InterleukinsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recom

NCBI and Uniprot Product Information

• NCBI GI #: 88014736
• NCBI GeneID: 16878
• NCBI Accession #: NP_001034626.1
• NCBI GenBank Nucleotide #: NM_001039537.2
• UniProt Accession #: P09056
• Molecular Weight: Calculated MW of 20.7 kDa with no tags. The predicted N-terminus is Ser 24. DTT-reduced Protein migrates as 33-45 kDa due to different glycosylation.
• NCBI Official Full Name: leukemia inhibitory factor isoform b
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: Lif
• NCBI Protein Information: leukemia inhibitory factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: Lif
• UniProt Synonym Gene Names: LIF; D factor
• UniProt Entry Name: LIF_MOUSE

Uniprot Description

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; maternal process involved in pregnancy; negative regulation of hormone secretion; leukemia inhibitory factor signaling pathway; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of adrenocorticotropic hormone secretion; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; astrocyte differentiation; regulation of cell differentiation; negative regulation of meiosis; positive regulation of cell proliferation; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; organ regeneration; stem cell maintenance; stem cell differentiation; positive regulation of peptidyl-serine phosphorylation; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; retina development in camera-type eye; neuron development; immune response; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; alveolus development; embryo implantation; lung development