LIF active protein

Human CellExp LIF, human recombinant

Cat. Num. AAA844523

• Gene Names: LIF; CDF; DIA; HILDA; MLPLI
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: LIF; Human CellExp LIF; human recombinant; CDF; DIA; HILDA; MLPLI; LIF active protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 88

• Applicable Applications for LIF active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug) by LAL method
• Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.
• Biological Activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for LIF active protein

Background: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Product Categories/Family for LIF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins (NEW) Cytokines; Growth Factors & Hormones; InterleukinsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recom

NCBI and Uniprot Product Information

• NCBI GI #: 380418324
• NCBI GeneID: 3976
• NCBI Accession #: NP_001244064.1
• NCBI GenBank Nucleotide #: NM_001257135.1
• UniProt Accession #: P15018; Q52LZ2; B2RCW7; B5MC23
• Molecular Weight: Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.
• NCBI Official Full Name: leukemia inhibitory factor isoform 2
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: LIF
• NCBI Official Synonym Symbols: CDF; DIA; HILDA; MLPLI
• NCBI Protein Information: leukemia inhibitory factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: LIF
• UniProt Synonym Gene Names: HILDA; LIF; D factor; MLPLI
• UniProt Entry Name: LIF_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of hormone secretion; multicellular organismal development; leukemia inhibitory factor signaling pathway; stem cell maintenance; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of meiosis; neuron development; blood vessel remodeling; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation; alveolus development; muscle morphogenesis; embryo implantation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of astrocyte differentiation