Mouse anti-Human Emilin-2 Monoclonal Antibody | anti-EMILIN2 antibody

Mouse Anti-Human Emilin-2

Cat. Num. AAA690727

• Gene Names: EMILIN2; FOAP-10; EMILIN-2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Protein G purified
• Synonyms: Emilin-2; Monoclonal Antibody; Mouse Anti-Human Emilin-2; Elastin microfibril interface-loctaed protein-2; anti-EMILIN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG_2b/kappa
• Clone Number: 828B3B3
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized; PBS
• Sequence Length: 1053

• Applicable Applications for anti-EMILIN2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: Use at 2-5 ug/ml; IF/IHC: IF (frozen sections)
• Immunogen: recombinant protein gC1q domain of Emilin-2
• Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using recombinant protein gC1q domain of Emilin-2.
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

Western Blot

(Western blot on recombinant protein obtained from supernatant of cells transduced with an Emilin-2 adenoviral construct.)

Immunofluorescence

(Immunofluorescence staining of human Emilin-2 (green) in human colon-rectal tissue (frozen section). Nuclear staining with TO-PRO3 in blue. Scale bar: 37.00 ?m.)

Related Product Information for anti-EMILIN2 antibody

Emilins (elastin microfibril interface located proteins) are extracellular matrix glycoproteins that localize to sites with proximity to elastin and microfibrils. They consist of an N-terminal cysteine-rich EMI domain and a conserved C-terminal gC1q-like domain. Emilin-1 is abundant in elastin-rich tissues such as blood vessels, skin, heart and lung. It influences placenta formation and initial organogenesis with a later role in interstitial connective tissue. Emilin-2 is larger than Emilin-1 and contains a unique proline-rich domain. It is likely involved in the process of elastogenesis. Multimerin-2 (also known as Emilin-3 or EndoGlyx-1) is expressed during embryonic development. Multimerin-1 (also known as Emilin-4) is expressed in platelets and the endothelium of blood vessels and may act as a carrier protein for platelet factor V. Emilin-5 is encoded by the Emilin-3 gene and is sometimes referred to as Emilin-3. It contains the N-terminal cysteine-rich EMI domain but lacks the C-terminal gC1q-like domain. Emilin-5 is expressed in human mesenchymal stem cells and plays an important role in skeletal development.

References

1. Doliana R et al, J Biol Chem 274:16773, 1999. 2. Mongiat M et al, J. Biol. Chem. 275:25471, 2000. 3. Doliana R et al, J Biol Chem 276:12003, 2001. 4. Braghetta P et al, Matrix Biol 21:603, 2002. 5. Spessotto P et al; J Biol Chem 278:6160, 2003. 6. Kishore U et al, Trends Immunol 25:551, 2004. 7. Verdone G et al, J Biomol NMR 29:91, 2004. 8. Zanetti M et al, Mol Cell Biol 24:638, 2004. 9. Mongiat et al, MCB 27:7176, 2007. 10. Mongiat et al, Neoplasia 12:294, 2010.

NCBI and Uniprot Product Information

• NCBI GI #: 60498978
• NCBI GeneID: 84034
• NCBI Accession #: NP_114437.2
• NCBI GenBank Nucleotide #: NM_032048.2
• UniProt Accession #: Q9BXX0; Q8NBH3; Q96JQ4; B2RMY3
• NCBI Official Full Name: EMILIN-2
• NCBI Official Synonym Full Names: elastin microfibril interfacer 2
• NCBI Official Symbol: EMILIN2
• NCBI Official Synonym Symbols: FOAP-10; EMILIN-2
• NCBI Protein Information: EMILIN-2; extracellular glycoprotein EMILIN-2; elastin microfibril interface-located protein 2
• UniProt Protein Name: EMILIN-2
• Protein Family: EMILIN
• UniProt Gene Name: EMILIN2
• UniProt Synonym Gene Names: Elastin microfibril interfacer 2
• UniProt Entry Name: EMIL2_HUMAN

Uniprot Description

Function: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

Subunit structure: Homotrimer associated through a moderately stable interaction of the C-terminal globular C1q domains, allowing the nucleation of the triple helix and then a further quaternary assembly to higher-order polymers via intermolecular disulfide bonds

By similarity. Interacts with EMILIN1.

Subcellular location: Secreted › extracellular space › extracellular matrix. Note: Found mainly at the interface between amorphous elastin and microfibrils.

Tissue specificity: Highest levels are present in fetal heart and adult lung. Intermediate levels in peripheral leukocytes, placenta, and spinal cord and low expression in fetal brain, spleen, thymus, and lung and in adult heart, aorta, testis, bone marrow, small intestine, thymus, lymph node, and appendix.

Sequence similarities: Contains 1 C1q domain.Contains 1 collagen-like domain.Contains 1 EMI domain.