Testing Data
LIF active protein
Mouse LIF
Reactivity
Mouse
Purity
> 98% by SDS-PAGE & silver stain
Synonyms
LIF; Mouse LIF; Recombinant Mouse Leukemia Inhibitory Factor (LIF); Differentiation-stimulating factor; Melanoma-derived LPL inhibitor; Emfilermin; LIF active protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 98% by SDS-PAGE & silver stain
Form/Format
Lyophilized
Sequence
N-Terminal Sequence: SPLPIT
Sequence Length
180
Endotoxin Level
< 0.1ng per ìg (IEU/ug) of rm LIF
Buffer
0.5X PBS
Preparation and Storage
The lyophilized LIF, though stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted LIF should be stored in working aliquots at -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for LIF active protein
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa.
Product Categories/Family for LIF active protein
References
1. Hirai H et al, Biochem J 438:11-23, 2011 2. Mathieu ME et al, Stem Cell Rev. 8:1-15, 2012 3. Metcalfe SM, Genes Immun 12(3):157-68, 2011 4. Broholm C and Pedersen BK, Exerc Immunol Rev 16:77-85, 2010 5. Aghajanova L, Curr Opin Obstet Gynecol 22(3):213-9, 2010 6. Paiva P et al, Cytokine Growth Factor Rev 20(4):319-28, 2009
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19.86 kDa
NCBI Official Full Name
leukemia inhibitory factor isoform b
NCBI Official Synonym Full Names
leukemia inhibitory factor
NCBI Official Symbol
Lif
NCBI Protein Information
leukemia inhibitory factor; d factor; differentiation-stimulating factor
UniProt Protein Name
Leukemia inhibitory factor
Protein Family
UniProt Gene Name
Lif
UniProt Synonym Gene Names
LIF; D factor
UniProt Entry Name
LIF_MOUSE
Uniprot Description
Function: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular location: Secreted.
Sequence similarities: Belongs to the LIF/OSM family.
Research Articles on LIF
Similar Products
Product Notes
The LIF lif (Catalog #AAA692107) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Mouse LIF reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: N-Terminal Sequence: SPLPIT. It is sometimes possible for the material contained within the vial of "LIF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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