SET7/9 recombinant protein

SET7/9 protein

Cat. Num. AAA836527

• Gene Names: SETD7; KMT7; SET7; SET9; SET7/9
• Applications: SDS-Page
• Purity: > 95% pure
• Synonyms: SET7/9; SET7/9 protein; EC 2.1.1.43 protein; SET-7 + SET-9; SET domain-containing protein 7 protein; SET7 + SET9; SET-7 + SET-9 protein; Histone H4-K4 methyltransferase protein; SET 7/9 protein; SET7 protein; SET D7 protein; KIAA1717 protein; Lysine N-methyltransferase 7 protein; SET 7/9; H4 lysine-4 specific protein; SET9 protein; H3 K4 HMTase protein; Histone H3 K4 methyltransferase protein; SET 9 protein; SET-7/9 protein; Histone H3 lysine 4 specific methyltransferase protein; SET-7/9; Histone H3-K4 methyltransferase SETD7 protein; SET domain-containing protein 8 protein; SET domain containing protein 7 protein; H3-K4-HMTase SETD7 protein; SET7 + SET9 protein; KMT7 protein; SETD7 protein; Histone-lysine N-methyltransferase SETD7 protein; Histone lysine N methyltransferase H3 lysine 4 specific SET7 protein; Lysine methyltransferase protein; SET domain-containing protein 7 FLJ21193 protein; Histone-lysine N-methyltransferase protein; SET7/9 Histone methyltransferase protein; SET 7 protein; SET7/9 recombinant protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: Supplied as a liquid in 50mM Tris-HCl buffer, pH7.5, containing 0.2M NaCl, 5mM DTT, and 20% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK
• Sequence Length: 366

• Applicable Applications for SET7/9 recombinant protein: SDS-PAGE
• Protein Type: Recombinant
• Biological Significance: Set 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Figure annotation denotes ug of protein loaded and % gel used.)

Related Product Information for SET7/9 recombinant protein

Purified recombinant Human SET7/9 protein

Product Categories/Family for SET7/9 recombinant protein

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 111309205
• NCBI GeneID: 80854
• UniProt Accession #: Q8WTS6; Q0VAH3; Q4W5A9; Q9C0E6; B5WWL3
• Molecular Weight: 40.7 kDa (366 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: SET domain containing (lysine methyltransferase) 7
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 7
• NCBI Official Symbol: SETD7
• NCBI Official Synonym Symbols: KMT7; SET7; SET9; SET7/9
• NCBI Protein Information: histone-lysine N-methyltransferase SETD7
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD7
• UniProt Gene Name: SETD7
• UniProt Synonym Gene Names: KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
• UniProt Entry Name: SETD7_HUMAN

Uniprot Description

SETD7: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Interacts with IPF1/PDX-1. Widely expressed. Expressed in pancreatic islets. Belongs to the histone-lysine methyltransferase family. SET7 subfamily.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: nucleoplasm; nucleolus; chromosome

Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; chromatin modification; response to DNA damage stimulus