MICA recombinant protein

MICA Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8121147

• Purity: >90% as determined by SDS-PAGE
• Synonyms: MICA; MICA Protein; Human; Recombinant (ECD; hFc Tag); Human MICA Protein (ECD; Fc Tag); DAMA-345G11.2 Protein; FLJ36918 Protein; FLJ60820 Protein; MGC111087 Protein; MGC21250 Protein; MIC-A Protein; PERB11.1 Protein; MHC class I polypeptide-related sequence A; MICA recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln308

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu 24
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human MICA (AAH16929.1)(Met1-Gln308) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MICA recombinant protein

Background: MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to the MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gamma-delta T cells, CD8+ alpha-beta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by the ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gamma-delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.

References

Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106.Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8.Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4.Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9.Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.

NCBI and Uniprot Product Information

• NCBI GI #: 16877353
• NCBI GeneID: 4276
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence A
• Protein Family: MICAL-like protein