Rabbit anti-Human TLT-1/TREML1 Polyclonal Antibody | anti-TREML1 antibody

Anti-TLT-1/TREML1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105998

• Gene Names: TREML1; TLT1; TLT-1; PRO3438; GLTL1825; dJ238O23.3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: TLT-1/TREML1; Polyclonal Antibody; Anti-TLT-1/TREML1 Antibody; Rabbit Polyclonal; TLT-1/TREML1 Antibody; Rabbit PAb; Antigen Affinity Purified; triggering receptor expressed on myeloid cells like 1; Anti-dJ238O23.3 Antibody; Anti-GLTL1825 Antibody; Anti-MGC119173 Antibody; Anti-PRO3438 Antibody; Anti-TLT-1 Antibody; Anti-TLT1 Antibody; Anti-UNQ1825/PRO3438 Antibody; anti-TREML1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TLT-1/TREML1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TREML1 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:200-1:1000; ICC/IF: 1:50-1:200
• Immunogen: Recombinant Human TLT-1/TREML1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human TLT-1/TREML1 (rh TLT-1/TREML1). TLT-1/TREML1 specific IgG was purified by Human TLT-1/TREML1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human TREML1 in human spleen with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of TREML1 in jurkat cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TREML1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and cell membrane.)

Related Product Information for anti-TREML1 antibody

Trem-like transcript 1 protein, also known as Triggering receptor expressed on myeloid cells-like protein 1, TREML1 and TLT-1, is a cytoplasm and single-pass type I membrane protein. TREML1/TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1/TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain, its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte/macrophages, and dendritic cells, via their association with DAP12. TREML1/TLT-1 is prepackaged, along with CD62P, into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1/TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury.

Product Categories/Family for anti-TREML1 antibody

ITIM/ITAM Immunoreceptors and Related Molecules

References

Washington A.V., et al., 2002, Blood 100:3822-4. Allcock R.J.N., et al., 2003, Eur. J. Immunol. 33:567-77. Washington A.V., et al., 2004, Blood 104:1042-7. Barrow A.D., et al., 2004, J. Immunol. 172:5838-42. Gattis J.L., et al., 2006, J. Biol. Chem. 281:13396-403.

NCBI and Uniprot Product Information

• NCBI GI #: 424036738
• NCBI GeneID: 340205
• NCBI Accession #: NP_001258736.1
• NCBI GenBank Nucleotide #: NM_001271807.1
• UniProt Accession #: Q86YW5; Q496B3; Q8IWY1; Q8IWY2
• Molecular Weight: 20,731 Da
• NCBI Official Full Name: trem-like transcript 1 protein isoform b
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells like 1
• NCBI Official Symbol: TREML1
• NCBI Official Synonym Symbols: TLT1; TLT-1; PRO3438; GLTL1825; dJ238O23.3
• NCBI Protein Information: trem-like transcript 1 protein
• UniProt Protein Name: Trem-like transcript 1 protein
• Protein Family: Trem-like transcript 1 protein
• UniProt Gene Name: TREML1
• UniProt Synonym Gene Names: TLT1; TLT-1
• UniProt Entry Name: TRML1_HUMAN

NCBI Description

This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. The encoded protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012]

Uniprot Description

TREML1: Cell surface receptor that may play a role in the innate and adaptive immune response. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: plasma membrane

Biological Process: regulation of immune response