Rat HMG box-containing protein 1 ELISA Kit | Hbp1 elisa kit

Rat HMG box-containing protein 1 ELISA Kit

Cat. Num. AAA2884767

• Gene Names: Hbp1; Hmgb1
• Reactivity: Rat
• Synonyms: HMG box-containing protein 1; Rat HMG box-containing protein 1 ELISA Kit; HMG box transcription factor 1; High mobility group box transcription factor 1; Hbp1; Hbp1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural ratHMG box-containing protein 1. No significant cross-reactivity or interference was observed.
• Sequence Length: 523

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.157 ng/mL
• Intra-Assay CV: <=4.8%
• Inter-Assay CV: <=6.7%
• Recovery: 86%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hbp1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofratHMG box-containing protein 1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMG box-containing protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHMG box-containing protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HMG box-containing protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HMG box-containing protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 270047512
• NCBI GeneID: 27080
• NCBI Accession #: NP_037353.2
• NCBI GenBank Nucleotide #: NM_013221.2
• UniProt Accession #: Q62661; Q5BK86
• Molecular Weight: 57,430 Da
• NCBI Official Full Name: HMG box-containing protein 1
• NCBI Official Synonym Full Names: HMG-box transcription factor 1
• NCBI Official Symbol: Hbp1
• NCBI Official Synonym Symbols: Hmgb1
• NCBI Protein Information: HMG box-containing protein 1
• UniProt Protein Name: HMG box-containing protein 1
• Protein Family: HMG box-containing protein
• UniProt Gene Name: Hbp1
• UniProt Entry Name: HBP1_RAT

NCBI Description

a transcriptional regulator that may play a central role in the stabilization and/or assembly of several multifunctional complexes through protein-protein interactions [RGD, Feb 2006]

Uniprot Description

HBP1: Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; RNA binding

Biological Process: positive regulation of potassium ion transport; regulation of transcription, DNA-dependent; transcription, DNA-dependent; Wnt receptor signaling pathway