Mouse High mobility group protein B1 ELISA Kit | Hmgb1 elisa kit

Mouse High mobility group protein B1 ELISA Kit

Cat. Num. AAA2882189

• Gene Names: Hmgb1; p30; Hmg1; HMG-1; SBP-1
• Reactivity: Mouse
• Synonyms: High mobility group protein B1; Mouse High mobility group protein B1 ELISA Kit; High mobility group protein 1; HMG-1; Hmgb1; Hmg-1; Hmg1; Hmgb1 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse High mobility group protein B1
• Sequence Length: 215

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156 ng/mL -10 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=7.1%
• Inter-assay Precision: Inter-Assay CV: <=9.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hmgb1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 926657659
• NCBI GeneID: 15289
• NCBI Accession #: NP_001300823.1
• NCBI GenBank Nucleotide #: NM_001313894.1
• UniProt Accession #: P63158; P07155; P27109; P27428
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: Hmgb1
• NCBI Official Synonym Symbols: p30; Hmg1; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmgb1
• UniProt Synonym Gene Names: Hmg-1; Hmg1; HMG-1
• UniProt Entry Name: HMGB1_MOUSE

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Cellular Component: cytoplasm; extracellular space; neuron projection; nucleus

Molecular Function: bent DNA binding; crossed form four-way junction DNA binding; cytokine activity; open form four-way junction DNA binding; protein kinase activator activity

Biological Process: DNA geometric change; eye development; induction of positive chemotaxis; lung development; positive regulation of cell migration; positive regulation of glycogen catabolic process; positive regulation of mesenchymal cell proliferation; positive regulation of mitotic cell cycle; positive regulation of myeloid cell differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to glucocorticoid stimulus