Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myogenin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myogenin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myogenin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Myogenin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Protein type: DNA-binding; Transcription factor
Cellular Component: nucleus
Molecular Function: chromatin DNA binding; DNA binding; protein binding; protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity
Biological Process: cell cycle; cell differentiation; mRNA transcription from RNA polymerase II promoter; multicellular organismal development; muscle development; myotube differentiation; negative regulation of cell proliferation; negative regulation of glycolysis; ossification; positive regulation of muscle atrophy; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of satellite cell proliferation; regulation of transcription, DNA-dependent; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; skeletal muscle fiber development; striated muscle atrophy; transcription, DNA-dependent
Research Articles on Myog
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Product Notes
The Mouse Myog myog (Catalog #AAA2886162) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886162 ELISA Kit recognizes Mouse Myog. It is sometimes possible for the material contained within the vial of "Myogenin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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