Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 ELISA Kit | Ddah1 elisa kit

Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 ELISA Kit

Cat. Num. AAA2885493

• Reactivity: Rat
• Synonyms: N(G); N(G)-dimethylarginine dimethylaminohydrolase 1; Rat N(G); N(G)-dimethylarginine dimethylaminohydrolase 1 ELISA Kit; DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1; Ddah1; Ddah; 3.5.3.18; Ddah1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat DDAH-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 285

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.182 ng/mL
• Intra-Assay CV: <=4.8%
• Inter-Assay CV: <=8.1%
• Recovery: 98%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ddah1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat DDAH-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDAH-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DDAH-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DDAH-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DDAH-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11560131
• NCBI GeneID: 64157
• NCBI Accession #: NP_071633.1
• NCBI GenBank Nucleotide #: NM_022297.2
• UniProt Accession #: O08557
• Molecular Weight: 31,426 Da
• NCBI Official Full Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• NCBI Official Synonym Full Names: dimethylarginine dimethylaminohydrolase 1
• NCBI Official Symbol: Ddah1
• NCBI Protein Information: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• UniProt Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• Protein Family: N(G),N(G)-dimethylarginine dimethylaminohydrolase
• UniProt Gene Name: Ddah1
• UniProt Synonym Gene Names: Ddah; DDAH-1; Dimethylarginine dimethylaminohydrolase 1
• UniProt Entry Name: DDAH1_RAT

NCBI Description

enzyme metabolizes dimethylarginine; involved in regulating the concentration of nitric oxide [RGD, Feb 2006]

Uniprot Description

DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.18; Hydrolase

Cellular Component: mitochondrion

Molecular Function: amino acid binding; dimethylargininase activity; metal ion binding

Biological Process: arginine metabolic process; citrulline metabolic process; positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure