Typical Testing Data/Standard Curve (for reference only)
Rat Glycogen Synthase Kinase 3 Beta ELISA Kit | GSK-3beta elisa kit
Rat Glycogen Synthase Kinase 3 Beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GSK3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSK3beta antibody and an GSK3beta-HRP conjugate. The assay sample and buffer are incubated together with GSK3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSK3beta concentration since GSK3beta from samples and GSK3beta-HRP conjugate compete for the anti-GSK3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by GSK3beta from the sample, fewer sites are left to bind GSK3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSK3beta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation
Protein type: EC 2.7.11.1; Kinase, protein; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily
Chromosomal Location of Human Ortholog: 3q13.3
Cellular Component: nucleoplasm; centrosome; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic spine; dendritic shaft; ribonucleoprotein complex; nucleus; cytosol; beta-catenin destruction complex; lipid raft
Molecular Function: integrin binding; ionotropic glutamate receptor binding; NF-kappaB binding; protein serine/threonine kinase activity; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; tau protein binding; protein kinase binding; ATP binding
Biological Process: fat cell differentiation; glycogen metabolic process; circadian rhythm; axon guidance; negative regulation of MAP kinase activity; genetic imprinting; re-entry into mitotic cell cycle; nerve growth factor receptor signaling pathway; positive regulation of protein binding; positive regulation of axon extension; response to lithium ion; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; peptidyl-serine phosphorylation; organ morphogenesis; negative regulation of neuron maturation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity
Research Articles on GSK-3beta
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Product Notes
The Rat GSK-3beta gsk3b (Catalog #AAA7251608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7251608 ELISA Kit recognizes Rat GSK-3beta. It is sometimes possible for the material contained within the vial of "Glycogen Synthase Kinase 3 Beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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