Mouse Glycogen Synthase Kinase 3 Beta ELISA Kit | GSK3beta elisa kit

Mouse GSK3beta (Glycogen Synthase Kinase 3 Beta) ELISA Kit

Cat. Num. AAA2533470

• Reactivity: Mouse
• Synonyms: Glycogen Synthase Kinase 3 Beta; Mouse GSK3beta (Glycogen Synthase Kinase 3 Beta) ELISA Kit; GSK3beta elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse GSK3beta in samples. No significant cross-reactivity or interference between Mouse GSK3beta and analogues was observed.
• Sequence Length: 28

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GSK3beta were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GSK3beta were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSK3beta elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse GSK3beta concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GSK3beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GSK3beta and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GSK3beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GSK3beta. You can calculate the concentration of Mouse GSK3beta in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta, partial
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Kinase, protein; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendritic shaft; dendritic spine; growth cone; lipid raft; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex

Molecular Function: ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; ubiquitin protein ligase binding

Biological Process: aging; axon guidance; cell migration; circadian rhythm; epidermal growth factor receptor signaling pathway; epithelial to mesenchymal transition; ER overload response; establishment of cell polarity; fat cell differentiation; fibroblast growth factor receptor signaling pathway; genetic imprinting; glycogen metabolic process; hippocampus development; innate immune response; myoblast fusion; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of glycogen biosynthetic process; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; nerve growth factor receptor signaling pathway; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; response to activity; response to drug; response to estradiol stimulus; response to insulin stimulus; Wnt receptor signaling pathway through beta-catenin