Western Blot (WB)
(PR15B Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PR15B antibody detected the PR15B protein (arrow).)
Rabbit anti-Human PR15B Polyclonal Antibody | anti-PR15B antibody
PR15B, CT (PPP1R15B, Protein phosphatase 1 regulatory subunit 15B) (FITC)
Gene Names
PPP1R15B; CREP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PR15B; Polyclonal Antibody; CT (PPP1R15B; Protein phosphatase 1 regulatory subunit 15B) (FITC); PPP1R15B; Protein phosphatase 1 regulatory subunit 15B; anti-PR15B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-PR15B antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PR15B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593-622 amino acids from the C-terminal region of human PR15B.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PR15B Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PR15B antibody detected the PR15B protein (arrow).)
Western Blot (WB)
(PR15B Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PR15B antibody detected the PR15B protein (arrow).)
Immunohistochemistry (IHC)
(PR15B Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PR15B Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PR15B Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PR15B Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PR15B antibody
PPP1R15B promotes dephosphorylation of the transcription initiation factor EIF2-alpha (EIF2S1; MIM 603907) through recruitment of protein phosphatase-1 (PP1) catalytic subunits (see MIM 176875) (Harding et al., 2009 [PubMed 19181853]).[supplied by OMIM].
Product Categories/Family for anti-PR15B antibody
References
Harding, H.P., et al. Proc. Natl. Acad. Sci. U.S.A. 106(6):1832-1837(2009). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Ceulemans, H., et al. Bioessays 24(4):371-381(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79152
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 15B
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 15B
NCBI Official Symbol
PPP1R15B
NCBI Official Synonym Symbols
CREP
NCBI Protein Information
protein phosphatase 1 regulatory subunit 15B
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 15B
UniProt Gene Name
PPP1R15B
UniProt Entry Name
PR15B_HUMAN
NCBI Description
PPP1R15B promotes dephosphorylation of the transcription initiation factor EIF2-alpha (EIF2S1; MIM 603907) through recruitment of protein phosphatase-1 (PP1) catalytic subunits (see MIM 176875) (Harding et al., 2009 [PubMed 19181853]).[supplied by OMIM, Feb 2010]
Uniprot Description
PPP1R15B: Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1. Belongs to the PPP1R15 family.
Protein type: Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: protein phosphatase type 1 complex
Molecular Function: protein binding; protein serine/threonine phosphatase activity
Biological Process: regulation of translation; response to hydrogen peroxide; ER overload response
Research Articles on PR15B
1. the mammalian traffic machinery co-opts p-eIF2alpha and CReP, regulators of translation initiation.
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Product Notes
The PR15B ppp1r15b (Catalog #AAA6336855) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PR15B, CT (PPP1R15B, Protein phosphatase 1 regulatory subunit 15B) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PR15B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PR15B ppp1r15b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PR15B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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