Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit | GSK3b elisa kit

Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit

Cat. Num. AAA2020422

• Reactivity: Rat
• Synonyms: Glycogen Synthase Kinase 3 Beta (GSK3b); Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit; Serine/threonine-protein kinase GSK3B; GSK3b elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycogen Synthase Kinase 3 Beta (GSK3b).; No significant cross-reactivity or interference between Glycogen Synthase Kinase 3 Beta (GSK3b) and analogues was observed.
• Sequence Length: 28

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Beta (GSK3b) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycogen Synthase Kinase 3 Beta (GSK3b) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSK3b elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Synthase Kinase 3 Beta (GSK3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Synthase Kinase 3 Beta (GSK3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Synthase Kinase 3 Beta (GSK3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Synthase Kinase 3 Beta (GSK3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GSK3b elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta, partial
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; Kinase, protein; Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; ribonucleoprotein complex; cytosol; beta-catenin destruction complex; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; NF-kappaB binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: glycogen metabolic process; circadian rhythm; fat cell differentiation; axon guidance; negative regulation of MAP kinase activity; genetic imprinting; re-entry into mitotic cell cycle; nerve growth factor receptor signaling pathway; positive regulation of protein binding; response to lithium ion; positive regulation of axon extension; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; peptidyl-serine phosphorylation; negative regulation of neuron maturation; organ morphogenesis; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity