General Cortisol ELISA Kit

Cortisol ELISA Kit

Cat. Num. AAA8005732

• Reactivity: General
• Applications: ELISA
• Synonyms: Cortisol; Cortisol ELISA Kit; Quantitative colorimetric detection of cortisol; (11beta)-11; 17; 21-trihydroxypregn-4-ene-3; 20-dione EIA Kit; Cortisol elisa kit

• Reactivity: General

• Applicable Applications for Cortisol elisa kit: ELISA (EIA)
• Intra-assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay.
• Inter-assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators.
• Preparation and Storage: Store at 4 degree C; Shipping Temperature: Blue Ice

Typical Testing Data/Standard Curve (for reference only)

(Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) StressXpress - SKT-201. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 - 3200 pg/ml.)

Testing Data

(Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.)

Related Product Information for Cortisol elisa kit

Background/Introduction: Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the "stress hormone" as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance1. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization2. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin1,3. Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer4, depression5, and schizophrenia6. It is already known that abnormal levels of cortisol are involved in Cushing's Syndrome and Addision's disease7.

Product Categories/Family for Cortisol elisa kit

Cell Signaling; Cancer; Oxidative Stress; Neuroscience; Assay Kits

References

1. E. Friess, et al., Eur J Clin Invest, 2000, 30, Suppl 3:46-50.
2. Freeman, Scott, 200
2. Biological Science. Prentice Hall; 2nd Pkg edition (December 30, 2004).
3. C. Longscope., J. Endocrinology, 1996,, Suppl S125-S127.
4. J. Herbert, Lancet, 1995 345, 1193-1194.
5. A. Michael, et al., Biol. Psychiatry, 2000, 48, 989-95.
6. C.R. Dequet and D.J. Wallace, Current Opin. Ivest. Drugs, 2001, 8, 1045-53.
7. W.M. Jeffries, Med. Hypotheses, 1998, 51, 114-4.