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Typical Testing Data/Standard Curve (for reference only)
(Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® - SKT-126. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM.)
Nitrotyrosine ELISA Kit
Nitrotyrosine ELISA Kit
Reactivity
Species Independent
Synonyms
Nitrotyrosine; Nitrotyrosine ELISA Kit; Nitro tyrosine ELISA Kit; 3-Nitrotyrosine ELISA Kit; 3-Nitro-L-tyrosine ELISA Kit; 3 Nitrotyrosine ELISA Kit; 3-NT ELISA Kit; 3NT ELISA Kit; Nitrotyrosine elisa kit
Reactivity
Species Independent
Specificity
ELISA kit used to quantitate Nitrotyrosine in samples.
Assay Type
Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Samples
Plasma, Serum, Cell Lysates, Urine
Detection Range
62.5 - 8000 nM
Sensitivity
50 nM
Assay Overview
1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 uL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 uL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25 degree C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 uL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 uL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Detection Method
Colorimetric Assay
Intra-Assay Precision
Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%.
Inter-Assay Precision
Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Target
Nitrotyrosine
Platform
Microplate
Preparation and Storage
Store at 4 degree C
Typical Testing Data/Standard Curve (for reference only)
(Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® - SKT-126. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM.)
Typical Testing Data/Standard Curve (for reference only)
(Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® - SKT-126. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM.)
Testing Data
(Diagram of the Competitive ELISA)
Testing Data
(Diagram of the Competitive ELISA)
Testing Data
(Diagram of the Preparation of Nitrotyrosine Standards)
Testing Data
(Diagram of the Preparation of Nitrotyrosine Standards)
Testing Data
(Diagram of the Triplicate Sample Plate Format)
Testing Data
(Diagram of the Triplicate Sample Plate Format)
Related Product Information for Nitrotyrosine elisa kit
Description: Colorimetric detection of Nitrotyrosine
Scientific Background: Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Scientific Background: Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Product Categories/Family for Nitrotyrosine elisa kit
Similar Products
Product Notes
The Species Independent Nitrotyrosine (Catalog #AAA807887) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA807887 ELISA Kit recognizes Species Independent Nitrotyrosine. It is sometimes possible for the material contained within the vial of "Nitrotyrosine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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