Typical Testing Data/Standard Curve (for reference only)
Cortisol ELISA Kit
Cortisol (human / mouse / rat) ELISA Kit
Reactivity
Human, Mouse, Rat
Synonyms
Cortisol; Cortisol (human / mouse / rat) ELISA Kit; Cortisol elisa kit
Reactivity
Human, Mouse, Rat
Application Notes
Quantitative detection of cortisol
Samples
Serum and plasma, Cell Culture Supernatant
Assay Type
Competitive
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to measure cortisol
Sensitivity: 1 ng/ml and detection range: 5 ng/ml to 480 ng/ml
Sensitivity: 1 ng/ml and detection range: 5 ng/ml to 480 ng/ml
Preparation and Storage
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cortisol elisa kit
Product Information: Cortisol (human/mouse/rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human, mouse, rat cortisol. Sensitivity: 1 ng/ml and detection range: 5 ng/ml to 480 ng/ml. 100 assays.
Background: MyBioSource's Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in serum and plasma. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). The amount of Cortisol present in serum undergoes diurnal variation, with the highest levels present in the early morning and lower levels at night. MyBioSource's Cortisol ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample or a HRP molecule which has Cortisol covalently attached to it is bound to the wells by immobilized antibody in a competitive manner. After a simultaneous incubation, the excess reagents are washed and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 5 ng/ml to 480 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Human, mouse, rat
Application- Quantitative detection of Cortisol
Background: MyBioSource's Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in serum and plasma. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). The amount of Cortisol present in serum undergoes diurnal variation, with the highest levels present in the early morning and lower levels at night. MyBioSource's Cortisol ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample or a HRP molecule which has Cortisol covalently attached to it is bound to the wells by immobilized antibody in a competitive manner. After a simultaneous incubation, the excess reagents are washed and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 5 ng/ml to 480 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Human, mouse, rat
Application- Quantitative detection of Cortisol
Product Categories/Family for Cortisol elisa kit
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Product Notes
The Human, Mouse, Rat Cortisol (Catalog #AAA843477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843477 ELISA Kit recognizes Human, Mouse, Rat Cortisol. Quantitative detection of cortisol. Researchers should empirically determine the suitability of the Cortisol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortisol, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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