Western Blot (WB)
(Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody. MBS9200045 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Mouse TBB5 Monoclonal Antibody | anti-TUBB antibody
TBB5 Antibody
IHC~~1:10~50
IF~~1:25
Western Blot (WB)
(Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody. MBS9200045 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody. MBS9200045 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. MBS9200045 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. MBS9200045 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunofluorescence (IF)
(Immunofluorescent analysis of A549 cells, using TBB5 Antibody. MBS9200045 was diluted at 1?25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of A549 cells, using TBB5 Antibody. MBS9200045 was diluted at 1?25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Uniprot Description
TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p21.33
Cellular Component: microtubule; cytoskeleton; nuclear envelope lumen; nucleus; cytosol
Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; MHC class I protein binding; ubiquitin protein ligase binding; protein complex binding; GTPase activating protein binding; structural molecule activity
Biological Process: protein polymerization; cell division; natural killer cell mediated cytotoxicity; metabolic process; organelle organization and biogenesis; cytoskeleton-dependent intracellular transport; spindle assembly; mitotic cell cycle; cell motility; G2/M transition of mitotic cell cycle; cellular process; microtubule-based process
Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6
Research Articles on TUBB
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Product Notes
The TUBB tubb (Catalog #AAA9200045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TBB5 Antibody reacts with Human, mouse, rat (Predicted Reactivity: Bovine, Hamster, Pig, Rat, Xenopus, Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's TBB5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000 IHC~~1:10~50 IF~~1:25. Researchers should empirically determine the suitability of the TUBB tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBB5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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