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Typical Testing Data/Standard Curve (for reference only)

Human inter-alpha (globulin) inhibitor H3 ELISA Kit | ITIH3 elisa kit

Human Inter-alpha-trypsin inhibitor heavy chain H3, ITIH3 ELISA Kit

Gene Names
ITIH3; H3P
Reactivity
Human
Synonyms
inter-alpha (globulin) inhibitor H3; Human Inter-alpha-trypsin inhibitor heavy chain H3; ITIH3 ELISA Kit; Human Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) ELISA kit; H3P; inter-alpha (globulin) inhibitor; H3 polypeptide; inter-alpha-trypsin inhibitor heavy chain H3; pre-alpha (globulin) inhibitor; ITIH3 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ITIH3. No significant cross-reactivity or interference between Human ITIH3 and analogues was observed.
Sequence Length
890
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
78.13 ng/ml-5000 ng/ml
Sensitivity
19.53 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ITIH3 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ITIH3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ITIH3. The competitive inhibition reaction is launched between with pre-coated ITIH3 and ITIH3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ITIH3 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
99,849 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 3
NCBI Official Symbol
ITIH3
NCBI Official Synonym Symbols
H3P
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H3; SHAP; ITI-HC3; ITI heavy chain H3; inter-alpha (globulin) inhibitor H3; inter-alpha-inhibitor heavy chain 3; serum-derived hyaluronan-associated protein; pre-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha (globulin) inhibitor, H3 polypeptide
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
UniProt Gene Name
ITIH3
UniProt Synonym Gene Names
ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP
UniProt Entry Name
ITIH3_HUMAN

NCBI Description

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]

Uniprot Description

ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process

Research Articles on ITIH3

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Product Notes

The Human ITIH3 itih3 (Catalog #AAA902708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA902708 ELISA Kit recognizes Human ITIH3. It is sometimes possible for the material contained within the vial of "inter-alpha (globulin) inhibitor H3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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