Human group-specific component (vitamin D binding protein) ELISA Kit | GC elisa kit

Human Vitamin D-binding protein, DBP ELISA Kit

Cat. Num. AAA702288

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• Reactivity: Human
• Synonyms: group-specific component (vitamin D binding protein); Human Vitamin D-binding protein; DBP ELISA Kit; DBP; DBP/GC; GRD3; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein; GC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DBP. No significant cross-reactivity or interference between human DBP and analogues was observed.
• Sequence Length: 474

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.156 mug/ml -10 mug/ml.
• Sensitivity: The minimum detectable dose of human DBP is typically less than 0.039 mug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GC elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DBP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for DBP. The competitive inhibition reaction is launched between with pre-coated DBP and DBP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DBP in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 32483410
• NCBI GeneID: 2638
• NCBI Accession #: NP_000574.2
• NCBI GenBank Nucleotide #: NM_000583.3
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 52,964 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 1
• NCBI Official Synonym Full Names: group-specific component (vitamin D binding protein)
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein; VDB; gc-globulin; vitamin D-binding alpha-globulin; epididymis secretory protein Li 51
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

Function: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.