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Typical Testing Data/Standard Curve (for reference only)

Mouse coagulation factor III, FIII ELISA Kit | F elisa kit

Mouse coagulation factor III, FIII ELISA Kit

Gene Names
F3; TF; Cf3; Cf-3; CD142; AA409063
Reactivity
Mouse
Synonyms
coagulation factor III; FIII; Mouse coagulation factor III; FIII ELISA Kit; CD142; TF; TFA; tissue factor; F; F elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse FIII. No significant cross-reactivity or interference between Mouse FIII and analogues was observed.
Sequence Length
294
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
7.8 pg/ml-500 pg/ml
Sensitivity
1.95 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Blood Coagulation
Protein Biological Process 3
Blood coagulation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for F elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FIII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FIII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FIII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FIII bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,935 Da
NCBI Official Full Name
tissue factor
NCBI Official Synonym Full Names
coagulation factor III
NCBI Official Symbol
F3
NCBI Official Synonym Symbols
TF; Cf3; Cf-3; CD142; AA409063
NCBI Protein Information
tissue factor
UniProt Protein Name
Tissue factor
UniProt Gene Name
F3
UniProt Synonym Gene Names
Cf-3; Cf3; TF
UniProt Entry Name
TF_MOUSE

NCBI Description

This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]

Uniprot Description

Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Research Articles on F

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Product Notes

The Mouse F f3 (Catalog #AAA706253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA706253 ELISA Kit recognizes Mouse F. It is sometimes possible for the material contained within the vial of "coagulation factor III, FIII, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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