Rabbit anti-Human, Mouse PAF65alpha Polyclonal Antibody | anti-PAF65alpha antibody

PAF65alpha (Q53) Polyclonal Antibody

Cat. Num. AAA3002235

• Gene Names: TAF6L; PAF65A
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PAF65alpha; Polyclonal Antibody; PAF65alpha (Q53) Polyclonal Antibody; TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; PCAF-associated factor 65-alpha; PAF65-alpha; TAF6L; PAF65A; anti-PAF65alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAF65alpha (Q53) polyclonal antibody detects endogenous levels of PAF65alpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 622

• Applicable Applications for anti-PAF65alpha antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human PAF65alpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PAF65alpha (Q53) Antibody at 1:1000 dilutionLane1:HepG2 whole cell lysate (10ug) Lane2:HEK293T whole cell lysate (10ug) Lane3:A549 whole cell lysate (10ug) Lane4:EC9706 whole cell lysate (10ug) Lane5:The Lung tissue lysate of Mouse (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PAF65alpha (Q53) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.)

Related Product Information for anti-PAF65alpha antibody

In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors. TAF6L, also known as PAF65A, is a 622 amino acid nuclear protein that functions as part of the PCAF (p300/CBP-associated factor) histone acetylase complex.

NCBI and Uniprot Product Information

• NCBI GI #: 5453844
• NCBI GeneID: 10629
• NCBI Accession #: NP_006464.1
• NCBI GenBank Nucleotide #: NP_006464.1
• UniProt Accession #: Q9Y6J9
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 6 like
• NCBI Official Symbol: TAF6L
• NCBI Official Synonym Symbols: PAF65A
• NCBI Protein Information: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
• UniProt Protein Name: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
• UniProt Gene Name: TAF6L
• UniProt Synonym Gene Names: PAF65A; PAF65-alpha
• UniProt Entry Name: TAF6L_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF6L: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Belongs to the TAF6 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: histone deacetylase complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription factor activity; transcription initiation