Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CHUK antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human IKKalpha Polyclonal Antibody | anti-CHUK antibody
IKKalpha Rabbit pab Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity purification
Synonyms
IKKalpha; Polyclonal Antibody; IKKalpha Rabbit pab Antibody; CHUK; IKKa ; CHUK ; IKBKA; IKK-alpha ; IKK1 ; IKKA ; NFKBIKA ; TCF16 ; Inhibitor of nuclear factor kappa-B kinase subunit alpha ; Transcription factor 16 ;; anti-CHUK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
745
Applicable Applications for anti-CHUK antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot (WB): 1/500-1000
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunoprecipitation (IP): 1/25-50
Flow Cytometry (FC/FACS): 1/10-50
Immunohistochemistry (IHC): 1/50-100
Immunocytochemistry (ICC): 1/50-100
Immunoprecipitation (IP): 1/25-50
Flow Cytometry (FC/FACS): 1/10-50
Immunogen
A synthetic -peptide of human IKKalpha
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CHUK antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CHUK antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-CHUK antibody
Rabbit polyclonal to Human IKKalpha
The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase and IKKgamma serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKbeta (Ser176 and Ser180 in IKKalpha), which causes conformational changes resulting in kinase activation (10-13).
The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase and IKKgamma serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKbeta (Ser176 and Ser180 in IKKalpha), which causes conformational changes resulting in kinase activation (10-13).
References
1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. 2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. 3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. 4. Brown, K. et al. (1995) Science 267, 1485-8. 5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7. Chen, Z.J. et al. (1996) Cell 84, 853-62. 8. Zandi, E. et al. (1997) Cell 91, 243-52. 9. Karin, M. (1999) Oncogene 18, 6867-74. 10. DiDonato, J.A. et al. (1997) Nature 388, 548-54. 11. Mercurio, F. et al. (1997) Science 278, 860-6. 12. Johnson, L.N. et al. (1996) Cell 85, 149-58. 13. Delhase, M. et al. (1999) Science 284, 309-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
745
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit alpha
NCBI Official Synonym Full Names
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol
CHUK
NCBI Official Synonym Symbols
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Protein Family
UniProt Gene Name
CHUK
UniProt Synonym Gene Names
IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
UniProt Entry Name
IKKA_HUMAN
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Product Notes
The CHUK chuk (Catalog #AAA125554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IKKalpha Rabbit pab Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IKKalpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot (WB): 1/500-1000 Immunohistochemistry (IHC): 1/50-100 Immunocytochemistry (ICC): 1/50-100 Immunoprecipitation (IP): 1/25-50 Flow Cytometry (FC/FACS): 1/10-50. Researchers should empirically determine the suitability of the CHUK chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKKalpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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