Typical Testing Data/Standard Curve (for reference only)
Nitric Oxide Assay Kit
Nitric Oxide Fluorometric Assay Kit
Synonyms
Nitric Oxide; Nitric Oxide Fluorometric Assay Kit; Nitrogen Oxide Fluorometric Assay Kit; Nitric Oxide assay kit
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes only 2-3 hours
Fast and convenient
The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate.
Fast and convenient
The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Nitric Oxide assay kit
Product Information: Nitric Oxide Fluorometric Assay Kit: Accurate & convenient Assay for Measurement of Nitric Oxide from Culture Media, Urine, Plasma, Serum & Tissue homogenates. Detection Method: Fluorescence. 100 Assays
Background: Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO?) and nitrate (NO??), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. MyBioSource's Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.
Summary: Detection method- Fluorescence (Ex/Em 380/461 nm)
Species reactivity- Mammalian
Applications- Measurement of total nitrate/nitrite concentration
Background: Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO?) and nitrate (NO??), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. MyBioSource's Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.
Summary: Detection method- Fluorescence (Ex/Em 380/461 nm)
Species reactivity- Mammalian
Applications- Measurement of total nitrate/nitrite concentration
Product Categories/Family for Nitric Oxide assay kit
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Product Notes
The Nitric Oxide (Catalog #AAA841654) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nitric Oxide, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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