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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human VGF Nerve Growth Factor Inducible (VGF) Polyclonal Antibody | anti-VGF antibody

Polyclonal Antibody to VGF Nerve Growth Factor Inducible (VGF)

Gene Names
VGF; SCG7; SgVII
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
VGF Nerve Growth Factor Inducible (VGF); Polyclonal Antibody; Polyclonal Antibody to VGF Nerve Growth Factor Inducible (VGF); anti-VGF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against VGF. It has been selected for its ability to recognize VGF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.45mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF-D LLLQYLLQGG ARQRGLGGRG LQEAAEERES AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS QEETPGHRRK EAEGTEEGGE EEDDEEMDP
Sequence Length
615
Applicable Applications for anti-VGF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant VGF (Asp330~Pro449) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052062
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded stomach tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded stomach tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Pancreas Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,258 Da
NCBI Official Full Name
neurosecretory protein VGF
NCBI Official Synonym Full Names
VGF nerve growth factor inducible
NCBI Official Symbol
VGF
NCBI Official Synonym Symbols
SCG7; SgVII
NCBI Protein Information
neurosecretory protein VGF
UniProt Protein Name
Neurosecretory protein VGF
Protein Family
UniProt Gene Name
VGF
UniProt Synonym Gene Names
NERP-1; NERP-2

NCBI Description

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cytoplasmic vesicle; endoplasmic reticulum lumen; extracellular space; intracellular membrane-bound organelle; transport vesicle

Molecular Function: growth factor activity; hormone activity; neuropeptide hormone activity

Biological Process: carbohydrate homeostasis; cellular protein metabolic process; defense response to bacterium; generation of precursor metabolites and energy; glucose homeostasis; insulin secretion; ovarian follicle development; post-translational protein modification; regulation of synaptic plasticity; response to cAMP; response to cold; response to dietary excess; response to insulin stimulus; sexual reproduction

Research Articles on VGF

Similar Products

Product Notes

The VGF vgf (Catalog #AAA2002663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to VGF Nerve Growth Factor Inducible (VGF) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VGF Nerve Growth Factor Inducible (VGF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VGF vgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF-D LLLQYLLQGG ARQRGLGGRG LQEAAEERES AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS QEETPGHRRK EAEGTEEGGE EEDDEEMDP. It is sometimes possible for the material contained within the vial of "VGF Nerve Growth Factor Inducible (VGF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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