Testing Data
VNN2 elisa pair set
VNN2 Matched ELISA Antibody Pair Set, Human
Synonyms
VNN2; VNN2 Matched ELISA Antibody Pair Set; Human; Human VNN2/Vanin-2 ELISA Pair Set; vanin 2; FOAP-4 Matched ELISA Antibody Pair Set; GPI-80 Matched ELISA Antibody Pair Set; VNN2 elisa pair set
Specificity
Quantitative determination of Human VNN2
Assay Type
Solid Phase Sandwich ELISA
Detection Range
156.25-10000pg/mL
Sensitivity
156.25pg/ml
Materials Provided
1. Capture Antibody 0.4 mg/mL of mouse anti-VNN2 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-VNN2 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 200 ng of recombinant VNN2. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-VNN2 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 200 ng of recombinant VNN2. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for VNN2 elisa pair set
Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-8, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein that belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.
This VNN2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human VNN2. It contains Human VNN2 capture antibody, Human VNN2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human VNN2 protein.
This VNN2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human VNN2. It contains Human VNN2 capture antibody, Human VNN2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human VNN2 protein.
References
Suzuki K. et al., 1999, J. Immunol. 162: 4277-84. Martin, F. et al., 2001, Immunogenetics. 53 (4):296-306. Liu T.et al., 2005, J. Proteome Res. 4: 2070-80. Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.
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Product Notes
The VNN2 (Catalog #AAA8118398) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "VNN2, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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