Neuropilin-2 ELISA Pair Set | NRP2 elisa pair set

Neuropilin-2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118288

• Synonyms: Neuropilin-2; Neuropilin-2 Matched ELISA Antibody Pair Set; Human; Human Neuropilin-2/NRP2 ELISA Pair Set; neuropilin 2; NP2 Matched ELISA Antibody Pair Set; NPN2 Matched ELISA Antibody Pair Set; PRO2714 Matched ELISA Antibody Pair Set; VEGF165R2 Matched ELISA Antibody Pair Set; NRP2 elisa pair set

• Specificity: Quantitative determination of Human Neuropilin-2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 156.25-10000pg/mL
• Sensitivity: 156.25pg/ml
• Materials Provided: 1. Capture Antibody 0.25 mg/mL of mouse anti-NRP2 monoclonal antibody, Dilute to a working concentration of 2.0ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL mouse anti-NRP2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 200 ng of recombinant NRP2. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for NRP2 elisa pair set

Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classI¢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as the formation of small lymphatic vessels and capillaries. A recent study has identified NRP-2 as a polysialylation protein expressed in human dendritic cells and modulates DC-T Cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis, and metastasis.

This Neuropilin-2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Neuropilin-2. It contains Human Neuropilin-2 capture antibody, Human Neuropilin-2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Neuropilin-2 protein.

References

Chen H. et al., 1997, Neuron. 19: 547-59. Bielenberg DR. et al., 2006, Exp Cell Res. 312: 584-93. Yuan L. et al., 2002, Development. 129: 4797-806. Benoit F. et al., 2006, Blood. 108: 1243-50. Sabrina C. et al., 2007, J Biol Chem. 282: 30346-56. Gray MJ. et al., 2008, J Natl Cancer Inst. 100: 109-20.