VNN2 recombinant protein

VNN2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120954

• Gene Names: VNN2; FOAP-4; GPI-80
• Purity: >98% as determined by SDS-PAGE
• Synonyms: VNN2; VNN2 Protein; Human; Recombinant (His Tag); Human VNN2/Vanin-2 Protein (His Tag); FOAP-4 Protein; GPI-80 Protein; vanin 2; VNN2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser492

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human VNN2 isoform 1 (NP_004656.2) without the propeptide (Met 1-Ser 492) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VNN2 recombinant protein

Background: Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-8, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein that belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.

References

Suzuki K. et al., 1999, J. Immunol. 162: 4277-84. Martin, F. et al., 2001,Immunogenetics. 53 (4):296-306. Liu T.et al., 2005, J. Proteome Res. 4: 2070-80. Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.

NCBI and Uniprot Product Information

• NCBI GI #: 17865814
• NCBI GeneID: 8875
• NCBI Accession #: NP_004656.2
• NCBI GenBank Nucleotide #: NM_004665.4
• UniProt Accession #: O95498; Q2XUN1; Q9UJF3; A0AUZ3; A6NDY1; A8K4E3; A8K7W0; B2DFZ0; B2DFZ1; B2DFZ2; B2DFZ3; F6XL73
• Molecular Weight: 52,844 Da
• NCBI Official Full Name: vascular non-inflammatory molecule 2 isoform 1
• NCBI Official Synonym Full Names: vanin 2
• NCBI Official Symbol: VNN2
• NCBI Official Synonym Symbols: FOAP-4; GPI-80
• NCBI Protein Information: vascular non-inflammatory molecule 2
• UniProt Protein Name: Vascular non-inflammatory molecule 2
• Protein Family: Vascular non-inflammatory molecule
• UniProt Gene Name: VNN2
• UniProt Synonym Gene Names: Vanin-2
• UniProt Entry Name: VNN2_HUMAN

NCBI Description

This gene product is a member of the Vanin family of proteins that share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. The encoded protein is a GPI-anchored cell surface molecule that plays a role in transendothelial migration of neutrophils. This gene lies in close proximity to, and in same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]