Western Blot (WB)
(Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.)
Rabbit His-Tag Polyclonal Antibody
His-Tag (Poly-His, hexa-HIS, hexa-Histidine) (Biotin)
Applications
Western Blot, ELISA
Purity
Antibody was purified by Protein A/G affinity chromatography prior to conjugation.
Synonyms
His-Tag; Polyclonal Antibody; His-Tag (Poly-His; hexa-HIS; hexa-Histidine) (Biotin); Poly-His; hexa-Histidine; anti-His-Tag antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes His-Tag
Purity/Purification
Antibody was purified by Protein A/G affinity chromatography prior to conjugation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-His-Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Poly-HIS peptide
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-His-Tag antibody
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Our anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Product Categories/Family for anti-His-Tag antibody
References
Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.
Similar Products
Product Notes
The His-Tag (Catalog #AAA6306650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
