Western Blot (WB)
(Anti-VNN2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa)
Rabbit anti-Human VNN2 Polyclonal Antibody | anti-VNN2 antibody
Anti-VNN2 Antibody, Rabbit Polyclonal
Gene Names
VNN2; FOAP-4; GPI-80
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
VNN2; Polyclonal Antibody; Anti-VNN2 Antibody; Rabbit Polyclonal; VNN2/Vanin-2 Antibody; Rabbit PAb; Antigen Affinity Purified; vanin 2; Anti-FOAP-4 Antibody; Anti-GPI-80 Antibody; anti-VNN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human VNN2/Vanin-2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-VNN2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Human VNN2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human VNN2 (rh VNN2). VNN2 specific IgG was purified by human VNN2 affinity chromatography
.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-VNN2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa)
Western Blot (WB)
(Anti-VNN2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa)
Related Product Information for anti-VNN2 antibody
Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-8, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein that belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.
References
Suzuki K. et al., 1999, J. Immunol. 162: 4277-84. Martin, F. et al., 2001, Immunogenetics. 53 (4):296-306. Liu T.et al., 2005, J. Proteome Res. 4: 2070-80. Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,844 Da
NCBI Official Full Name
vascular non-inflammatory molecule 2 isoform 1
NCBI Official Synonym Full Names
vanin 2
NCBI Official Symbol
VNN2
NCBI Official Synonym Symbols
FOAP-4; GPI-80
NCBI Protein Information
vascular non-inflammatory molecule 2
UniProt Protein Name
Vascular non-inflammatory molecule 2
Protein Family
UniProt Gene Name
VNN2
UniProt Synonym Gene Names
Vanin-2
UniProt Entry Name
VNN2_HUMAN
NCBI Description
This gene product is a member of the Vanin family of proteins that share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. The encoded protein is a GPI-anchored cell surface molecule that plays a role in transendothelial migration of neutrophils. This gene lies in close proximity to, and in same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]
Research Articles on VNN2
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Product Notes
The VNN2 vnn2 (Catalog #AAA8105746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VNN2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VNN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the VNN2 vnn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VNN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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