Testing Data
CNTN5 elisa pair set
CNTN5 Matched ELISA Antibody Pair Set, Human
Synonyms
CNTN5; CNTN5 Matched ELISA Antibody Pair Set; Human; Human Contactin 5/CNTN5 ELISA Pair Set; contactin 5; HNB-2s Matched ELISA Antibody Pair Set; NB-2 Matched ELISA Antibody Pair Set; CNTN5 elisa pair set
Specificity
Quantitative determination of Human CNTN5
Assay Type
Solid Phase Sandwich ELISA
Detection Range
62.5-4000pg/mL
Sensitivity
62.5pg/ml
Materials Provided
1. Capture Antibody 0.3 mg/mL of rabbit anti-CNTN5 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL mouse anti-CNTN5 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 200 ng of recombinant CNTN5. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL mouse anti-CNTN5 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 200 ng of recombinant CNTN5. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CNTN5 elisa pair set
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.
This CNTN5 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CNTN5. It contains Human CNTN5 capture antibody, Human CNTN5 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CNTN5 protein.
This CNTN5 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CNTN5. It contains Human CNTN5 capture antibody, Human CNTN5 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CNTN5 protein.
References
Kamei Y, et al. (2000) Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members. Genomics 69(1):113-9.Ogawa J, et al. (2001) Neural recognition molecule NB-2 of the contactin/F3 subgroup in rat: Specificity in neurite outgrowth-promoting activity and restricted expression in the brain regions. J Neurosci Res. 65(2):100-10.Li H, et al. (2003) Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2. Eur J Neurosci. 17(5):929-36.Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.Toyoshima M, et al. (2009) Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 513(4):349-62.
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Product Notes
The CNTN5 (Catalog #AAA8118267) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CNTN5, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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