Mouse anti-Human RELT/TNFRSF19L Monoclonal Antibody | anti-RELT antibody

Anti-RELT/TNFRSF19L Antibody, Mouse Monoclonal

Cat. Num. AAA8103139

• Gene Names: RELT; TRLT; TNFRSF19L
• Reactivity: Human
• Applications: Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: RELT/TNFRSF19L; Monoclonal Antibody; Anti-RELT/TNFRSF19L Antibody; Mouse Monoclonal; RELT/TNFRSF19L Antibody; Mouse MAb; RELT tumor necrosis factor receptor; Anti-TNFRSF19L Antibody; Anti-TRLT Antibody; anti-RELT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 01
• Specificity: Human RELT/TNFRSF19L
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RELT antibody: Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:20-1:100
• Immunogen: Recombinant Human RELT/TNFRSF19L protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELT/TNFRSF19L (rh RELT/TNFRSF19L). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Confocal immunofluorescence analysis of Human RELT in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human RELT monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm and plasma membrane.)

Related Product Information for anti-RELT antibody

Receptor expressed in lymphoid tissues (RELT), also known as tumor necrosis factor receptor superfamily, member 19-like (TNFRSF19L), is a member of the TNF-receptor superfamily. This receptor is especially abundant in hematologic tissues. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1. RELT/TNFRSF19L is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. RELT/TNFRSF19L is a type I transmembrane glycoprotein with a cysteine-rich extracellular domain, possessing significant homology to other members of the TNFR superfamily, especially TNFRSF19, DR3, OX40, and LTbeta receptor. RELT/TNFRSF19L is able to activate the NF-kappaB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. RELT/TNFRSF19L is able to activate the NF-kappaB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. Although the soluble form of RELT fusion protein does not inhibit the one-way mixed lymphocyte reaction, immobilized RELT/TNFRSF19L is capable of costimulating T-cell proliferation in the presence of CD3 signaling.

References

Sica GL, et al. (2001) RELT, a new member of the tumor necrosis factor receptor superfamily, is selectively expressed in hematopoietic tissues and activates transcription factor NF-kappaB. Blood. 97(9): 2702-7.Polek TC, et al. (2006) The TNF receptor, RELT, binds SPAK and uses it to mediate p38 and JNK activation. Biochem Biophys Res Commun. 343(1): 125-34.Cusick JK, et al. (2006) Identification of RELT homologues that associate with RELT and are phosphorylated by OSR1. Biochem Biophys Res Commun. 340(2): 535-43.

NCBI and Uniprot Product Information

• NCBI GI #: 21361873
• NCBI GeneID: 84957
• NCBI Accession #: NP_116260.2
• NCBI GenBank Nucleotide #: NM_032871.3
• UniProt Accession #: Q969Z4; Q86V34; Q96JU1; Q9BUX7
• Molecular Weight: 46,092 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 19L
• NCBI Official Synonym Full Names: RELT tumor necrosis factor receptor
• NCBI Official Symbol: RELT
• NCBI Official Synonym Symbols: TRLT; TNFRSF19L
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 19L; receptor expressed in lymphoid tissues
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 19L
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: RELT
• UniProt Synonym Gene Names: TNFRSF19L
• UniProt Entry Name: TR19L_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is especially abundant in hematologic tissues. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1 (TRAF1). This receptor is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

RELT: Mediates activation of NF-kappa-B. May play a role in T- cell activation. Belongs to the RELT family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: cytoplasm; integral to membrane; plasma membrane