Testing Data
METTL1 elisa pair set
METTL1 Matched ELISA Antibody Pair Set, Human
Synonyms
METTL1; METTL1 Matched ELISA Antibody Pair Set; Human; Human METTL1 ELISA Pair Set; methyltransferase like 1; C12orf1 Matched ELISA Antibody Pair Set; TRM8 Matched ELISA Antibody Pair Set; TRMT8 Matched ELISA Antibody Pair Set; YDL201w Matched ELISA Antibody Pair Set; METTL1 elisa pair set
Specificity
Quantitative determination of Human METTL1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
156.25-10000pg/mL
Sensitivity
156.25pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Human METTL1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human METTL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 182 ng of recombinant Human METTL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human METTL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 182 ng of recombinant Human METTL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for METTL1 elisa pair set
tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
This METTL1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human METTL1. It contains Human METTL1 capture antibody, Human METTL1 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human METTL1 protein.
This METTL1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human METTL1. It contains Human METTL1 capture antibody, Human METTL1 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human METTL1 protein.
Product Categories/Family for METTL1 elisa pair set
References
Bahr, A.et al., 1999, Genomics. 57 (3):424-8. Wikman, H. et al., 2005, Genes Chromosomes Cancer. 42 (2):193-9. Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.
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Product Notes
The METTL1 (Catalog #AAA8118378) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "METTL1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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