METTL1 recombinant protein

METTL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120840

• Gene Names: METTL1; TRM8; TRMT8; C12orf1; YDL201w
• Purity: >95% as determined by SDS-PAGE
• Synonyms: METTL1; METTL1 Protein; Human; Recombinant (His Tag); Human METTL1 Protein (His Tag); C12orf1 Protein; TRM8 Protein; TRMT8 Protein; YDL201w Protein; methyltransferase like 1; METTL1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 0.5M NaCl, 20% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp32-Gln265

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human METTL1 (NP_005362.3)(Asp 32-Gln 265) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for METTL1 recombinant protein

Background: tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Product Categories/Family for METTL1 recombinant protein

MAPK-Erk Pathway

References

Bahr, A.et al., 1999, Genomics. 57 (3):424-8. Wikman, H. et al., 2005,Genes Chromosomes Cancer. 42 (2):193-9. Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.

NCBI and Uniprot Product Information

• NCBI GI #: 167004078
• NCBI GeneID: 4234
• NCBI Accession #: NP_005362.3
• NCBI GenBank Nucleotide #: NM_005371.5
• UniProt Accession #: Q9UBP6; Q14105; Q53FS9; B2RBX1; H7BXF2
• Molecular Weight: 17,423 Da
• NCBI Official Full Name: tRNA (guanine-N(7)-)-methyltransferase isoform a
• NCBI Official Synonym Full Names: methyltransferase like 1
• NCBI Official Symbol: METTL1
• NCBI Official Synonym Symbols: TRM8; TRMT8; C12orf1; YDL201w
• NCBI Protein Information: tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
• UniProt Protein Name: tRNA (guanine-N(7)-)-methyltransferase
• UniProt Gene Name: METTL1
• UniProt Entry Name: TRMB_HUMAN

NCBI Description

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

METTL1: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Belongs to the methyltransferase superfamily. TrmB family.

Protein type: Methyltransferase; EC 2.1.1.33; RNA processing

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; tRNA (guanine-N7-)-methyltransferase activity; tRNA binding

Biological Process: tRNA modification; tRNA methylation