DDC elisa pair set

DDC Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118276

• Synonyms: DDC; DDC Matched ELISA Antibody Pair Set; Human; Human DOPA Decarboxylase/DDC ELISA Pair Set; dopa decarboxylase (aromatic L-amino acid decarboxylase); AADC Matched ELISA Antibody Pair Set; DDC elisa pair set

• Specificity: Quantitative determination of Human DDC

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 156.25-10000pg/mL
• Sensitivity: 156.25pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human DOPA Decarboxylase/DDC monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human DOPA Decarboxylase/DDC monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.2ug/mL in Dilution Buffer before use. ; 3. Standard Each vial contains 400 ng of recombinant Human DOPA Decarboxylase/DDC. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 10000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for DDC elisa pair set

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3, 4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

This DDC Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human DDC. It contains Human DDC capture antibody, Human DDC detector antibody (HRP) and a highly purified Baculovirus-Insect-expressed recombinant Human DDC protein.

Product Categories/Family for DDC elisa pair set

Neurotransmitter Associated Enzymes

References

Ichinose, H. et al., 1989, Biochem. Biophys. Res. Commun. 164: 1024-1030. Lisa, J. S. et al., 1992, Genomics 13: 469-471. Moore, P. S. et al., 1996, Biochem. J. 315:249-256. Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47. Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823. Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.