Rabbit anti-Human METTL1 Polyclonal Antibody | anti-METTL1 antibody

Anti-METTL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105388

• Gene Names: METTL1; TRM8; TRMT8; C12orf1; YDL201w
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: METTL1; Polyclonal Antibody; Anti-METTL1 Antibody; Rabbit Polyclonal; METTL1 Antibody; Rabbit PAb; Antigen Affinity Purified; methyltransferase like 1; Anti-C12orf1 Antibody; Anti-TRM8 Antibody; Anti-TRMT8 Antibody; Anti-YDL201w Antibody; anti-METTL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human METTL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-METTL1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human METTL1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human METTL1 (rh METTL1). METTL1 specific IgG was purified by Human METTL1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human METTL1 in human bladder carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein.)

Immunoprecipitation (IP)

(METTL1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-METTL1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-METTL1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)

Western Blot (WB)

(Anti-METTL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-METTL1 antibody

tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Product Categories/Family for anti-METTL1 antibody

MAPK-Erk Pathway

References

Bahr, A.et al., 1999, Genomics. 57 (3):424-8. Wikman, H. et al., 2005, Genes Chromosomes Cancer. 42 (2):193-9. Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.

NCBI and Uniprot Product Information

• NCBI GI #: 167004078
• NCBI GeneID: 4234
• NCBI Accession #: NP_005362.3
• NCBI GenBank Nucleotide #: NM_005371.5
• UniProt Accession #: Q9UBP6; Q14105; Q53FS9; B2RBX1; H7BXF2
• Molecular Weight: 17,423 Da
• NCBI Official Full Name: tRNA (guanine-N(7)-)-methyltransferase isoform a
• NCBI Official Synonym Full Names: methyltransferase like 1
• NCBI Official Symbol: METTL1
• NCBI Official Synonym Symbols: TRM8; TRMT8; C12orf1; YDL201w
• NCBI Protein Information: tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
• UniProt Protein Name: tRNA (guanine-N(7)-)-methyltransferase
• UniProt Gene Name: METTL1
• UniProt Entry Name: TRMB_HUMAN

NCBI Description

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

METTL1: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Belongs to the methyltransferase superfamily. TrmB family.

Protein type: Methyltransferase; EC 2.1.1.33; RNA processing

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; tRNA (guanine-N7-)-methyltransferase activity; tRNA binding

Biological Process: tRNA modification; tRNA methylation