Human Angiotensin II Receptor 2 antibody, AT2R-Ab ELISA Kit | AGTR2 elisa kit

Human Angiotensin II Receptor 2 antibody, AT2R-Ab ELISA Kit

Cat. Num. AAA701565

• Gene Names: AGTR2; AT2; ATGR2; MRX88
• Reactivity: Human
• Synonyms: Angiotensin II Receptor 2 antibody; AT2R-Ab; Human Angiotensin II Receptor 2 antibody; AT2R-Ab ELISA Kit; AGTR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AT2R-Ab. No significant cross-reactivity or interference between human AT2R-Ab and analogues was observed.
• Sequence Length: 363

• Samples: Serum, plasma
• Assay Type: Sandwich
• Detection Range: 0.312 U/ml -20 U/ml.
• Sensitivity: The minimum detectable dose of human AT2R-Ab is typically less than 0.078 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGTR2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AT2R. Standards and samples are pipetted into the wells with a biotin-conjugated AT2R and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AT2R-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 607812
• NCBI GeneID: 186
• UniProt Accession #: P50052; Q13016; Q6FGY7; B2R9V1; AAB34100
• Molecular Weight: 41,184 Da
• NCBI Official Full Name: angiotensin II receptor type 2 subtype
• NCBI Official Synonym Full Names: angiotensin II receptor, type 2
• NCBI Official Symbol: AGTR2
• NCBI Official Synonym Symbols: AT2; ATGR2; MRX88
• NCBI Protein Information: type-2 angiotensin II receptor; angiotensin II type-2 receptor
• UniProt Protein Name: Type-2 angiotensin II receptor
• Protein Family: Type-2 angiotensin II receptor
• UniProt Gene Name: AGTR2
• UniProt Synonym Gene Names: AT2
• UniProt Entry Name: AGTR2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor 1 family, and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus, but scantily in adult tissues, except brain, adrenal medulla, and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked mental retardation. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.1 PublicationManual assertion based on experiment in:Ref.18