LMAN2 elisa pair set

LMAN2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118432

• Synonyms: LMAN2; LMAN2 Matched ELISA Antibody Pair Set; Human; Human LMAN2/VIP36 ELISA Pair Set; lectin; mannose binding 2; C5orf8 Matched ELISA Antibody Pair Set; GP36B Matched ELISA Antibody Pair Set; VIP36 Matched ELISA Antibody Pair Set; LMAN2 elisa pair set

• Specificity: Quantitative determination of Human LMAN2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 31.25pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-LMAN2 monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.25 mg/mL mouse anti-LMAN2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 71 ng of recombinant LMAN2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LMAN2 elisa pair set

LMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively.

This LMAN2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LMAN2. It contains Human LMAN2 capture antibody, Human LMAN2 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human LMAN2 protein.

References

Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21.Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63.Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9.Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 (Pt 17): 2813-21.