Testing Data
Kininogen 1 ELISA Pair Set | KNG1 elisa pair set
Kininogen 1 Matched ELISA Antibody Pair Set, Human
Synonyms
Kininogen 1; Kininogen 1 Matched ELISA Antibody Pair Set; Human; Human KNG1/Kininogen 1 ELISA Pair Set; kininogen 1; BDK Matched ELISA Antibody Pair Set; BK Matched ELISA Antibody Pair Set; KNG Matched ELISA Antibody Pair Set; KNG1 elisa pair set
Specificity
Quantitative determination of Human Kininogen 1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
31.25-2000pg/mL
Sensitivity
31.25pg/ml
Materials Provided
1. Capture Antibody 0.15 mg/mL of mouse anti-KNG1 monoclonal antibody, Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL mouse anti-KNG1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 25 ng of recombinant KNG1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2ng/mL is recommended.
2. Detection Antibody 0.25 mg/mL mouse anti-KNG1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 25 ng of recombinant KNG1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for KNG1 elisa pair set
Kininogen-1, also known as high molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, KNG1, and BDK, is a secreted protein that contains three cystatin domains. Kininogen-1/KNG1 is a protein from the blood coagulation system as well as the kinin-kallikrein system. It is a protein that adsorbs to the surface of biomaterials that come in contact with blood. Kininogen-1/KNG1 circulates throughout the blood and quickly adsorbs to the material surfaces. Kininogen-1/KNG1 is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. Kininogen-1/KNG1 is one of the kininogens, a class of proteins. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed. When the clinical data were combined, it turned out that all patients had a deficiency of the same protein. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
This Kininogen 1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Kininogen 1. It contains Human Kininogen 1 capture antibody, Human Kininogen 1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Kininogen 1 protein.
This Kininogen 1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Kininogen 1. It contains Human Kininogen 1 capture antibody, Human Kininogen 1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Kininogen 1 protein.
Product Categories/Family for KNG1 elisa pair set
References
Ohkubo I., et al., (1984), Isolation of a human cDNA for alpha 2-thiol proteinase inhibitor and its identity with low molecular weight kininogen. Biochemistry 23:5691-5697.Takagaki Y., et al., (1985), Cloning and sequence analysis of cDNAs for human high molecular weight and low molecular weight prekininogens. Primary structures of two human prekininogens.J. Biol. Chem. 260:8601-8609.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.
Similar Products
Product Notes
The KNG1 (Catalog #AAA8118272) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Kininogen 1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
