LCN1 elisa pair set

LCN1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118384

• Synonyms: LCN1; LCN1 Matched ELISA Antibody Pair Set; Human; Human LCN1/Lipocalin 1 ELISA Pair Set; lipocalin 1; Lipocalin 1 Matched ELISA Antibody Pair Set; MGC71975 Matched ELISA Antibody Pair Set; PMFA Matched ELISA Antibody Pair Set; TLC Matched ELISA Antibody Pair Set; TP Matched ELISA Antibody Pair Set; VEGP Matched ELISA Antibody Pair Set; LCN1 elisa pair set

• Specificity: Quantitative determination of Human LCN1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 31.25pg/ml
• Materials Provided: 1. Capture Antibody 0.15 mg/mL of mouse anti-LCN1 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating. ; 2. Detection Antibody 0.25 mg/mL mouse anti-LCN1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 35 ng of recombinant LCN1. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LCN1 elisa pair set

Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear Prealbumin, VEGP, Tear lipocalin, and LCN1 is a secreted protein that belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1/VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1/VEGP is identical to lingual von Ebner's gland protein and is also produced in the prostate, nasal mucosa, and tracheal mucosa. Homologous proteins have been found in the rat, pig, and probably dog and horse. Lipocalin-1/VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1/VEGP acts as the principal lipid-binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1/VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1/LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1/LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.

This LCN1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LCN1. It contains Human LCN1 capture antibody, Human LCN1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LCN1 protein.

References

Lassagne H. et al., 1993, Exp. Eye Res. 56:605-609. Redl, B. et al., 2000, Biochim Biophys Acta 1482 (1-2):241-8. Wojnar P. et al., 2001, J. Biol. Chem. 276:20206-20212. Wojnar P. et al., 2003, J. Biol. Chem. 278:16209-16215. Breustedt D.A. et al., 2005, J. Biol. Chem. 280:484-493.